Hello, I prepare libraries for RNAseq for a GAIIx we have. I use both High sensitivity DNA chip and KAPA library quant kit to quantify my samples. However, I have noticed sometimes for some samples the concentrations are way off as when I put even 4 pm i do nnot get anything and it seems over clustered(too many clusters/mm2)
any suggestion or any kits/method I could reliably use so it does not have the limitation of not quantifying very highly concentrated samples?
Thanks a lot in advance
any suggestion or any kits/method I could reliably use so it does not have the limitation of not quantifying very highly concentrated samples?
Thanks a lot in advance
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