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  • help with TruSeq

    I have recently used TruSeq kit for making 8 of Brassica RNAseq libraries and after amplification (15 cycles) and running on the gel (1% Agarose) i found that 5 out of 8 libraries have a broader range of fragments (smear) compared to the rest of the 3 libraries. I don't know why and the only reason i can think of possible over-amplification. But if it is so why only 5 and not all 8 have this problem. Any help is appreciated.

    I have attached the picture of the gel for easy understanding.

    Thanks
    Attached Files

  • #2
    The picture you attach looks to my viewer (FoxIt pdf viewer) like it has been processed through some bizarre filter to make it look like a scanning electron micrograph. That is off-putting -- but also you don't give the marker sizes.

    --
    Phillip

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    • #3
      You might want to try repurifying them with the beads.

      Comment


      • #4
        Hi pmiguel, marker sizes added now.
        Attached Files

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