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Old 11-24-2010, 11:57 AM   #1
kirankm
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Location: Cardiff

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Default TruSeq adapter sequences

Hi all,
My aim is to do targeted sequencing.
I wish to use the TruSeq DNA library prep kit for multiplexing and then use the Roche-Nilmblegen sequence capture method.

As Illumina has changed the protocol by adding the indexes to the adapters, I guess the oligos used in the Roche protocol (Hybridisation enhancement oligos etc) need to be modified.

The problem I do not have access to the Illumina TruSeq sequences namely; adapter-index oligos and the primer sequences.

Can some one share these sequences?

Thanks
KiranKM
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Old 11-29-2010, 12:13 PM   #2
GW_OK
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I don't believe they are publically available. Our FAS says Illumina won't be providing them until sometime early next year, despite my protestations.
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Old 05-10-2012, 01:23 AM   #3
aleferna
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still haven't ... frustrated grunt, anybody has the adapters for the Nextera V2?
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Old 05-10-2012, 05:48 AM   #4
peromhc
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Quote:
Originally Posted by aleferna View Post
still haven't ... frustrated grunt, anybody has the adapters for the Nextera V2?
If you contact Illumina tech support, they will send them to you.
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Old 05-10-2012, 06:32 AM   #5
aleferna
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I did, but they sent me a "letter to customer" pdf and I can't quite understand it, they have a RNA primers, DNA primers, some for oligo some for genomic dna, its a bit cryptic and if I get the wrong sequence it will be terrible expensive mistake.
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