I am curious if anyone is currently using the Equalizer kit from Life Tech to prepare their libraries for templation. If so, are you bulking multiple libraries together, and are you finding that it does a good job of having somewhat equal amounts of reads per library?
We frequently put 96 AmpliSeq libraries together on one 318 chip. Our method of quantitation is by qPCR. The Equalizer kit has been recommended to me on multiple occasions but I have not yet heard if it works well on multiplexed runs. We are at a point where qPCR is a huge bottleneck and we need to purchase another qPCR machine or figure something else out.
Any insight or user experience is welcomed.
We frequently put 96 AmpliSeq libraries together on one 318 chip. Our method of quantitation is by qPCR. The Equalizer kit has been recommended to me on multiple occasions but I have not yet heard if it works well on multiplexed runs. We are at a point where qPCR is a huge bottleneck and we need to purchase another qPCR machine or figure something else out.
Any insight or user experience is welcomed.
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