Wondering if anyone is piloting NEB or Kapa library preparation kits for Illumina sequencing? Heard that the original Truseq library prep kit is being discontinued at the end of the year, and remaining kits are not useful for target capture. They are pushing toward using Nextera for these applications, but we tried it and had very poor luck, likely due to fragment size range after enzymatic fragmentation. Would love any pointers/input on using kits from these other companies.
Thanks.
Thanks.
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