Dear Colleagues. I'm looking at small RNA fragments in my samples associated with or derived from mRNAs. I prefer to use the running window generator in SeqMonk because I can then annotate against various features without having to redefine the probes. The question is, what are the best settings to use? The libraries are typical NEBNext generated from 18-21 and 23-30 nt size fractionated RNA. I'm not normalising the read counts but I am rejecting duplicate reads. All advice greatly appreciated.
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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04-22-2024, 07:01 AM -
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