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Old 04-23-2019, 05:26 PM   #1
tickteng
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Location: Japan

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Default Newbie: Primer with index

Hi everyone, kinda new to the whole sequencing. So I want to run multiple sample in minion. There is barcode provided by oxford nanopore, but i also would like to attach index to my pcr.

I am planning to make 18 or 22 bp of index.
The question is, is there any platform that you use to design index? or is it just randomly decided by you?
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Old 04-23-2019, 11:53 PM   #2
Marc_Jones
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You could just add the index to the primers when you order them.

or

SQK-RPB004 or SQK-PBK004 depending on your experiment.
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Old 04-24-2019, 12:18 AM   #3
tickteng
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hmm, not really like that. So beside the one provided by nanopore, I plan to attach some kind of index to the primer itself, so the result can be divided further in one barcode.
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