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Old 05-17-2012, 07:35 AM   #1
nupurgupta
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Location: New Jersey

Join Date: Aug 2010
Posts: 29
Default Command line blast with remote option

Hello,
I am trying to run blastn from my command line with the -remote option, and I get a proxy error as

Error: (311.26) SOCK#1000[6]@130.14.29.110:80: [SOCK::Connect] Failed pending connect(): Closed {error=111,Connection refused}
Error: (303.7) [URL_Connect] Failed to connect to www.ncbi.nlm.nih.gov:80: Closed
Error: (311.26) SOCK#2000[6]@130.14.29.110:80: [SOCK::Connect] Failed pending connect(): Closed {error=111,Connection refused}
...

Any idea how to fix this please?
Thanks
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Old 05-18-2012, 07:32 AM   #2
maubp
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It is a network problem, so it could go away if you retry at a less busy time. Can you use BLAST from your browser? Is there a proxy in the way? If so, you might need to talk to your network administrator.
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Old 05-18-2012, 07:47 AM   #3
nupurgupta
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It is a proxy error, I think. I was wondering if there is a proxy option in blastn itself. I can run it from the browser. But I can also run it directly from applications like clcBio. So the configuration can be done - just not sure how to do it for blastn.
Am getting my network people to look into it also..
Thanks so much!
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