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Old 11-07-2012, 07:07 AM   #1
kwaraska
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Location: Boston,MA

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Default DNA Extraction from Bone

Has anyone tried to extract DNA from bone and then successfully create an Illumina Library from it?

We have such material from a customer and it seems the concentrations are correct, and it shears appropriately, but we have tried twice (unsuccessfully) to create a library.

The customer says she used the "kit from Covaris".

Any thoughts?

Thanks in advance for any help
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Old 11-09-2012, 04:33 AM   #2
yaximik
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Default

I did. There are whole bunch of methods described in detail in papers from Paabo's lab. The most important is to use fluorescence-based DNA quantification methods, which preferentially detect dsDNA. I cannot comment more as DNA quality depends on too many factors when dealing with bones. The sample may have too much inhibitors, degraded, etc.
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