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Old 12-09-2016, 11:25 AM   #1
akhattri
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Location: Chicago ZIP Code 60637

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Default RNAseq of too degraded FFPE samples

I want to prepare RNAseq libraries with RNAs from FFPE samples. In the past I have used TruSeq Access kit and it worked well if DV200 was >30. As I have same samples that are too degraded I wonder if anyone has successfully prepared library using very low qualty samples like with DV200 around 15-20.

Thanks,
Arun
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