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Old 12-17-2013, 09:43 PM   #1
maoyanjun
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Post Cancer panel v2,low quality,316v2

Hi, these days ,I run some 316v2 chips on PGM, and the %low quality show very high, ranged from 27-40%! Can anyone tell me why?
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Old 01-03-2014, 03:15 AM   #2
JPC
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We run a Proton and found that we improved our quality by reducing the quantity of DNA that goes into the emPCR by by 20%, we now do this is standard

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Old 01-06-2014, 12:02 AM   #3
maoyanjun
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Quote:
Originally Posted by JPC View Post
We run a Proton and found that we improved our quality by reducing the quantity of DNA that goes into the emPCR by by 20%, we now do this is standard

JPC
Thank you for your reply!
We had try that before, but it didn't work!
by the way, how do you quantify the library, 2100 or qubit?
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Old 01-06-2014, 12:46 AM   #4
JPC
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qubit for quant, but we also use the bioanalyser tp make sure the profile looks as expected (no primer etc.)

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Old 01-12-2014, 07:27 PM   #5
maoyanjun
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qubit for quant, but we also use the bioanalyser tp make sure the profile looks as expected (no primer etc.)

JPC
thank you very much
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Old 02-19-2014, 06:38 AM   #6
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You probably need to check amplifiability of your DNA. Using the RNaseP kit will help out with that. Crap in, crap out.
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