Hypothetically
If I have 24 samples and I submit them to a facility to run my RNA-seq then can this happen?
The technician puts in varying amount of prepped library into the lanes so how does DESeq2 account for this variability in it's normalization?
If I put in 20 ng if DNA in lane one and 50 ng in lane 2 how is this accounted for by the software or is this something I should be aware of and account for by myself.
If I have 24 samples and I submit them to a facility to run my RNA-seq then can this happen?
The technician puts in varying amount of prepped library into the lanes so how does DESeq2 account for this variability in it's normalization?
If I put in 20 ng if DNA in lane one and 50 ng in lane 2 how is this accounted for by the software or is this something I should be aware of and account for by myself.
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