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Old 10-01-2014, 04:47 AM   #1
sinnafoch
Junior Member
 
Location: Austria

Join Date: Sep 2013
Posts: 9
Default Resequencing the same Illumina library several times

Hi,

I hope I am in the right forum. I have a Illumina library made of total RNA for a metatranscriptomics study.
If I sequence the library several times under the same conditions, can I expect (more or less) the same results? Same number of reads? Same distribution of reads across different taxa?

many thanks,

Philipp
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Old 10-01-2014, 05:22 AM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
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You can expect similar results, though you should expect some variation in read number around +/-10% which may affect quality of reads and hence results. For instance, if first sequencing run clustered in high side of recommended density and the subsequent run was clustered10% higher than first run that will affect quality adversely and consequently your analysis results. But if it clustered lower than first run you will get less reads possibly with higher quality and that is less likely to affect the results.
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