Hello,
I've been looking around for information on Illumina mate-pair library preps. Apparently paired-end contamination and chimeric reads are often produced.
In practice, how frequent are these mishaps? Does the operator have much leverage on these issues, or are they pre-determined by the mechanics of the process?
Thanks in advance,
Daniel
I've been looking around for information on Illumina mate-pair library preps. Apparently paired-end contamination and chimeric reads are often produced.
In practice, how frequent are these mishaps? Does the operator have much leverage on these issues, or are they pre-determined by the mechanics of the process?
Thanks in advance,
Daniel
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