Hi y'all, I left my complete sequencing reaction at room temp right outside the thermocycler by accident for about ~45 min b/c I was waiting for the lid to heat and forgot about it. It's the normal Big Dye terminator sequencing rxn, forward or reverse primer, big dye, water, sequencing buffer. I found my mistake and quickly put it into the PCR machine. DO you think it will be ok? I do. Of course BDTv3.1 is light sensitive but it was kinda in the shade, not under direct lights.. I hope 45 min isn't too long. Thanks!
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
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Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
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