Go Back   SEQanswers > Applications Forums > RNA Sequencing

Similar Threads
Thread Thread Starter Forum Replies Last Post
Sequencing data analysis of pair-end sequencing Smriti Illumina/Solexa 8 11-04-2011 07:51 AM
PubMed: Titration-free massively parallel pyrosequencing using trace amounts of start Newsbot! Literature Watch 3 05-06-2010 12:41 PM
pair-end sequencing produces single-end read artifact pparg Bioinformatics 9 03-29-2010 11:15 AM
A pair end sequencing paper Chien-Yuan Chen Literature Watch 0 03-14-2009 01:46 PM
Adequate coverage for genome wide pair end sequencing Chien-Yuan Chen Bioinformatics 0 03-07-2009 10:49 AM

Thread Tools
Old 03-02-2009, 09:59 AM   #1
Chien-Yuan Chen
Location: USA

Join Date: Feb 2009
Posts: 19
Default How many amounts of mRNA do you start the pair end sequencing?

How many amounts of mRNA do you start the pair end sequencing?

By the protocol, 10 ug RNA or 100ng mRNA will work. But by the published article, maybe 200 ng or more is necessary. It is not easy to collect the mRNA in clinical samples.
Chien-Yuan Chen is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 11:19 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO