SEQanswers

Go Back   SEQanswers > Applications Forums > RNA Sequencing



Similar Threads
Thread Thread Starter Forum Replies Last Post
Low RIN of plant RNA and high abundaces of rRNA. yonne Sample Prep / Library Generation 6 10-03-2013 12:22 PM
GPU vs Phi: advice needed yaximik Bioinformatics 4 09-11-2013 10:11 AM
Software advice needed regarding SNP calling Burger Bioinformatics 3 05-18-2012 05:24 AM
ChIP-seq advice needed bcm Illumina/Solexa 2 03-22-2010 04:22 AM
Low quantity of ChIP DNA- Advice? Quest Illumina/Solexa 0 01-29-2009 09:51 PM

Reply
 
Thread Tools
Old 07-15-2014, 03:48 PM   #1
ZoeG
Member
 
Location: California, US

Join Date: Jun 2013
Posts: 31
Default Advice needed: low RIN for miRNA sequencing

We are planing a miRNA sequencing for our mouse muscle samples.
Usually, we get very good RNA samples with our Qiagen miRNeasy kit, with RIN >8.
Unfortunatelly, for this batch, we have RIN ~5.0 for all samples.
A260/A280 is always around 2.0, which excludes contamination. The tissue is freshly dissected and stored in RNAlater at -20 C for 2 days. Then we extracted RNA using Qiagen miRNeasy kit.
It is some weird that we have such low RIN numbers.
Now, we need make a choice, to throw these RNA samples away or go forward for microRNA sequencing?

Anyone has similar experience?
Advice is appreciated.
ZoeG is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 04:13 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2019, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO