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Old 07-15-2014, 03:48 PM   #1
Location: California, US

Join Date: Jun 2013
Posts: 31
Default Advice needed: low RIN for miRNA sequencing

We are planing a miRNA sequencing for our mouse muscle samples.
Usually, we get very good RNA samples with our Qiagen miRNeasy kit, with RIN >8.
Unfortunatelly, for this batch, we have RIN ~5.0 for all samples.
A260/A280 is always around 2.0, which excludes contamination. The tissue is freshly dissected and stored in RNAlater at -20 C for 2 days. Then we extracted RNA using Qiagen miRNeasy kit.
It is some weird that we have such low RIN numbers.
Now, we need make a choice, to throw these RNA samples away or go forward for microRNA sequencing?

Anyone has similar experience?
Advice is appreciated.
ZoeG is offline   Reply With Quote

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