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Old 02-02-2016, 07:46 AM   #1
alrossi
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Location: São Paulo, Brazil

Join Date: Feb 2016
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Default Differential expression analysis using DESeq for TCGA data

Hi.

I’m analysing RNA Sequencing (RNAseq) data of Breast Invasive Carcinoma cases for tumor-normal matched samples downloaded from TCGA. An amount of 113 samples were retrieved. I’m interested in the matched tumor and normal samples to evaluate gene expression differences between invasive breast carcinoma tissue and its matched normal tissue.

I’m using with the raw_counts (...rsem.genes.result) as input of DESeq methods to perform differential expression analysis.

Should I use all genes raw counts as input of DESeq methods or I can use just the subset of genes I’m interested?

Here is the count read data: https://www.dropbox.com/s/niv6xk8bbp...GENES.csv?dl=0

Thank you.

André Rossi

Last edited by alrossi; 02-03-2016 at 05:36 AM. Reason: To post a link for the read count data.
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