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Old 12-07-2010, 02:45 AM   #1
Kanak Vaidya
Junior Member
 
Location: Gujarat

Join Date: Oct 2009
Posts: 2
Default Emulsion PCR for SOLiD Sequencing

Hi,

I am working on SOLiD Technology for quite some time now. During the P2 enriched beads preparation where a ring of P2 enriched beads onto glycerol layer appears, at times it happens that few of the templated beads are observed attached to the walls of microcentrifuge and i guess these contribute to the polyclonality. So do not ever collect these beads. Can anybody suggest why this happens and how to avoid such condition.

Thanks
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