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The NEXTflex™ 16S V4 Amplicon-Seq Kit has been developed to simplify bacterial metagenomics studies using Illumina® HiSeq and MiSeq platforms. This kit allows users to go from sample to sequence in two hours, making it the fastest library prep kit available.
Using specialized NEXTflex primers that target the V4 region of the 16S subunit, a single PCR amplification simultaneously ligates the necessary sequencing and barcoded region for multiplexing. The entire workflow requires only one clean-up step, maximizing recovery. Ideal for studies of microbiome community composition or comparative metagenomics, the NEXTflex V4 Amplicon-Seq Kit is available with up to 48 barcodes. Higher degrees of multiplexing are available on a custom basis. Contact [email protected] for more information.
Using specialized NEXTflex primers that target the V4 region of the 16S subunit, a single PCR amplification simultaneously ligates the necessary sequencing and barcoded region for multiplexing. The entire workflow requires only one clean-up step, maximizing recovery. Ideal for studies of microbiome community composition or comparative metagenomics, the NEXTflex V4 Amplicon-Seq Kit is available with up to 48 barcodes. Higher degrees of multiplexing are available on a custom basis. Contact [email protected] for more information.
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