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  • Genome to assemble- need hardware help!

    Hey Seqanswers- I'm in a bind,

    I'm soon going to be in receipt of Illumina Hi-seq PE genomic reads for a non-model plant with genome about 400Mb. I'm OK re software for de novo assembly, but my problem is that I don't have reliable hardware: I've been trying to use iPlant, but it's looking like their hardware is turning out to be unreliable (sorry to say) and I need to get these reads de novo assembled into a draft genome.

    I work at a small university with no computer core that can lend me a few hundred spare gigs of RAM, so I'm in need of other options. I'm new to this stuff in general, but I've heard about Galaxy- does Galaxy (like iPlant) have the capability to assemble WGS reads uploaded to their server?

    Any recommendations welcome!

  • #2
    Originally posted by NYGen View Post
    Hey Seqanswers- I'm in a bind,

    I'm soon going to be in receipt of Illumina Hi-seq PE genomic reads for a non-model plant with genome about 400Mb. I'm OK re software for de novo assembly, but my problem is that I don't have reliable hardware: I've been trying to use iPlant, but it's looking like their hardware is turning out to be unreliable (sorry to say) and I need to get these reads de novo assembled into a draft genome.

    I work at a small university with no computer core that can lend me a few hundred spare gigs of RAM, so I'm in need of other options. I'm new to this stuff in general, but I've heard about Galaxy- does Galaxy (like iPlant) have the capability to assemble WGS reads uploaded to their server?

    Any recommendations welcome!
    Sounds as if you are a good candidate for XSEDE
    Startup allocations are very straight forward and they have lots of available machines with large memory at the various sites.

    Just go to the web site, then select the User Services pulldown and ultimately, "Allocations".

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