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Hi, Adiza:
Based on my experiments with Ampure beads, the yield is around 50% -80% ( depends on the size you need). If you need the exact 300bp DNA, you may need to run the beads selection more than one time.
Our DNA library needs 150-300bp DNA and they are all fine for us. You know the ratio of beads applied would determine the size of DNA that would produce. For our lab, if I did the fragmentation of DNA firstly, I may get various of sizs( 50-500bp). There is always some smaller size or bigger size inside you don't need. I applied 1.2 times of beads to do the size selection . Since I only need DNA with 150-300bp. So you could do fragmentation, and then applied different ratio of beads to see which ratio works best for you. Hope that would help.
P.S: the system didn't allow me to send you message directly. So I post it here for you viewing.
Based on my experiments with Ampure beads, the yield is around 50% -80% ( depends on the size you need). If you need the exact 300bp DNA, you may need to run the beads selection more than one time.
Our DNA library needs 150-300bp DNA and they are all fine for us. You know the ratio of beads applied would determine the size of DNA that would produce. For our lab, if I did the fragmentation of DNA firstly, I may get various of sizs( 50-500bp). There is always some smaller size or bigger size inside you don't need. I applied 1.2 times of beads to do the size selection . Since I only need DNA with 150-300bp. So you could do fragmentation, and then applied different ratio of beads to see which ratio works best for you. Hope that would help.
P.S: the system didn't allow me to send you message directly. So I post it here for you viewing.
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