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Hi! everyone! Now i have 2 sRNA high-throughput seqencing library that include 1000,0000 clean reads, i want to search potential siRNA according to criterion that the two perfectly complementary sRNAs with 2 nt overhangs at the 3-end were considered to be siRNA. Tags from clean reads were aligned with each other by blast+,but blast is very slowly! Can anyone tell me some better pipeline or scripts such as perl ? i would be be deeply grateful!
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM