Hi all,
Quick question, did some shifting of a BAM file by first converting it into BED with bamtobed and shifting with an awk script, however when I converted it back to bam after the file size reduced by 1/3 from 750mb to 250mb. Although when I run samtools flagstat on both before and after it shows me 22 million reads in both the 750mb and 250mb files. Any ideas?
Quick question, did some shifting of a BAM file by first converting it into BED with bamtobed and shifting with an awk script, however when I converted it back to bam after the file size reduced by 1/3 from 750mb to 250mb. Although when I run samtools flagstat on both before and after it shows me 22 million reads in both the 750mb and 250mb files. Any ideas?
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