Hi,
My Background:
I am new to NGS/RNA-seq and have been doing some background research. Now I suppose I have the basic theoretical knowledge and some specific questions, which I hope someone could help me with.Although I have used bioinformatics tools extensively, I dont have any programming experience.
My workstation: 120g ssd/3 -TbHDD/16gRAM(upgrading to 32g)/6 core processor/OS: Bio-Linux 8.
My Aim: To Identify and analyze lncRNAs from Stem cells of specific cell sub population. I want to generate a pipeline for lncRNA identification using STAR (not TOP-Hat/I am aware of the Tuxedo Suit though).
I really dont know how to go about this, I could not find a pipeline using STAR specifically for the purpose. Any suggestions or tutorial links will be very helpful. Also, any great places to download stem cell/cancer cell RNA-Seq (fastq) files??
My Background:
I am new to NGS/RNA-seq and have been doing some background research. Now I suppose I have the basic theoretical knowledge and some specific questions, which I hope someone could help me with.Although I have used bioinformatics tools extensively, I dont have any programming experience.
My workstation: 120g ssd/3 -TbHDD/16gRAM(upgrading to 32g)/6 core processor/OS: Bio-Linux 8.
My Aim: To Identify and analyze lncRNAs from Stem cells of specific cell sub population. I want to generate a pipeline for lncRNA identification using STAR (not TOP-Hat/I am aware of the Tuxedo Suit though).
I really dont know how to go about this, I could not find a pipeline using STAR specifically for the purpose. Any suggestions or tutorial links will be very helpful. Also, any great places to download stem cell/cancer cell RNA-Seq (fastq) files??
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