Hi Everyone -
I've been given some RNA that was extracted from snap frozen mouse muscle (leg). The bioanalyzer tells me that the RNA is degraded (RIN ~2.5-3) with fragment sizes centered around 200-500 bases. Is it possible to make a library out of this? Ideally I'd like to ribodeplete the sample first.
Any thoughts appreciated.
I've been given some RNA that was extracted from snap frozen mouse muscle (leg). The bioanalyzer tells me that the RNA is degraded (RIN ~2.5-3) with fragment sizes centered around 200-500 bases. Is it possible to make a library out of this? Ideally I'd like to ribodeplete the sample first.
Any thoughts appreciated.
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