SMART™ mRNA Amplification Kit

[fearnoweevil]

Hi!

I was just wondering if anyone has ever used the SMART™ mRNA Amplification Kit to amplify mRNA and if you have any feedback on how well it works.

Thanks!

[SS00]

Interested in this too. I read this article comparing NuGen and SMART technologies for low-input RNA http://www.ncbi.nlm.nih.gov/pubmed/23685885

From what I understood, I laid out a summary:



The only drawback I could find to the SMART technology in the paper is the poor GC performance. Not sure if this has to do with the Clontech polymerase (Advtantage 2 Polymerase) or the Illumina Polymerase (Phusion or Truseq?) post-ligation? Anyone have experience with swapping these for a polymerase with better GC coverage?

I am trying to choose a protocol for RNA-seq with single cells so I am looking at pg amounts of DNA. Any advice/experiences would be appreciated!