During an attempt to isolate DNA from spores and save a days work I started to wonder about the contents of DNAdvance secret ingredients.
isolating DNA from spores does NOT work with this kit, i tried modifying the protocol by adding 0.5mm glass beads together with the lysis buffer proceeded by 3X20 seconds in a bead-beater incubation 20 min. at 60C after the glass beads settled on the bottom the supernatant was pipette out - followed by the rest of the original protocol. - did not work either.
however i would like to know the content of the lysis buffer and the Bind1 buffer and Bind2 buffer (besides beads)
any other recommendations in terms of DNA extraction from stubborn spores (using the kit) are of course appreciated
below is the only description given the manufacture
link to product protocol and details https://www.beckmancoulter.com/wsrpo...000151v001.pdf
Reagent-------------Description----------------Storage Condition Upon Arrival
Lysis Buffer-----------Lysis (clear)-------------------Room Temperature
Proteinase K----------Lyophilized Enzyme----------(-20°C)
Proteinase K Buffer--Proteinase K Buffer (clear)---Room Temperature
Bind1 Buffer----------Binding solution (clear)------Room Temperature
Bind2 Buffer----------Magnetic Solution------------(4°C)
Elution Buffer---------12mM Tris pH 8.0-------------Room Temperature
isolating DNA from spores does NOT work with this kit, i tried modifying the protocol by adding 0.5mm glass beads together with the lysis buffer proceeded by 3X20 seconds in a bead-beater incubation 20 min. at 60C after the glass beads settled on the bottom the supernatant was pipette out - followed by the rest of the original protocol. - did not work either.
however i would like to know the content of the lysis buffer and the Bind1 buffer and Bind2 buffer (besides beads)
any other recommendations in terms of DNA extraction from stubborn spores (using the kit) are of course appreciated
below is the only description given the manufacture
link to product protocol and details https://www.beckmancoulter.com/wsrpo...000151v001.pdf
Reagent-------------Description----------------Storage Condition Upon Arrival
Lysis Buffer-----------Lysis (clear)-------------------Room Temperature
Proteinase K----------Lyophilized Enzyme----------(-20°C)
Proteinase K Buffer--Proteinase K Buffer (clear)---Room Temperature
Bind1 Buffer----------Binding solution (clear)------Room Temperature
Bind2 Buffer----------Magnetic Solution------------(4°C)
Elution Buffer---------12mM Tris pH 8.0-------------Room Temperature
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