Hi everyone,
I'm puzzled: on the samplesheet for Nextera libraries, the chemistry is listed as "amplicon", yet we know that the transposase sequence at the 3' end of the adapters is going to require a different forward read primer. How does the MiSeq (which is the platform I'm using) distinguish between these two? The generated sample sheets seem nearly identical. Perhaps they just make a mix of the two primers together?
I'm interested in the answer for my own custom amplicon application. I know others have posted their approaches but I need to do something slightly different, but want the dual indexing.
Thanks for insights you can provide!
Evan
I'm puzzled: on the samplesheet for Nextera libraries, the chemistry is listed as "amplicon", yet we know that the transposase sequence at the 3' end of the adapters is going to require a different forward read primer. How does the MiSeq (which is the platform I'm using) distinguish between these two? The generated sample sheets seem nearly identical. Perhaps they just make a mix of the two primers together?
I'm interested in the answer for my own custom amplicon application. I know others have posted their approaches but I need to do something slightly different, but want the dual indexing.
Thanks for insights you can provide!
Evan