Hello,
I read about random hexamer priming bias in Illumina RNASEQ (see Hansen et al., NAR 2010, vol 38 no 12). Specifically, "There is a strong distinctive pattern in the nucleotide frequencies of the first 13 positions at the 50-end of mapped RNA-Seq reads".
I assume that is the reason why my FASTQC report does not show parallel lines in the first 13 positions for GC content.
How do you deal with it? Will trimming work?
Thanks!
I read about random hexamer priming bias in Illumina RNASEQ (see Hansen et al., NAR 2010, vol 38 no 12). Specifically, "There is a strong distinctive pattern in the nucleotide frequencies of the first 13 positions at the 50-end of mapped RNA-Seq reads".
I assume that is the reason why my FASTQC report does not show parallel lines in the first 13 positions for GC content.
How do you deal with it? Will trimming work?
Thanks!
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