Hello,
In the Q-PCR results from KAPA quantification of my libraries, I got extra-peaks in the melt curve graph and I can not figure out the reason. I have attached the image
- I used the TruSeq stranded total RNA sample preparation with the Ribo-Zero depletion and the BA didn´t show any anomaly (according to tehnical support the extra peaks could be adapter-dimer but that is not consistent with the BA profiles).
- The samples are primary cells from paraganglioma and glioblastoma tissue and, theoretically, they have presence of cytomegalovirus (that is what we want to confirm).
Does anyone have a similar experience and an explanation, please?
Thank you in advance for any help!
Anna.
In the Q-PCR results from KAPA quantification of my libraries, I got extra-peaks in the melt curve graph and I can not figure out the reason. I have attached the image
- I used the TruSeq stranded total RNA sample preparation with the Ribo-Zero depletion and the BA didn´t show any anomaly (according to tehnical support the extra peaks could be adapter-dimer but that is not consistent with the BA profiles).
- The samples are primary cells from paraganglioma and glioblastoma tissue and, theoretically, they have presence of cytomegalovirus (that is what we want to confirm).
Does anyone have a similar experience and an explanation, please?
Thank you in advance for any help!
Anna.
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