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Old 07-31-2014, 11:15 AM   #1
zoe561
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Default ATAC seq lysis time

Hello,

I was wondering if anyone has attempted the ATACseq protocol on primary mouse basal keratinocytes and has some idea of optimal lysis time for 50,000 cells?

Thanks!
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Old 10-13-2014, 12:46 AM   #2
Agun
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Hello, did you find the answer you needed?

I am also interested in ATAC-seq for our population of keratinocytes. Perhaps we can help eachother out.
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Old 11-13-2014, 01:13 PM   #3
obischof
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Quote:
Originally Posted by zoe561 View Post
Hello,

I was wondering if anyone has attempted the ATACseq protocol on primary mouse basal keratinocytes and has some idea of optimal lysis time for 50,000 cells?

Thanks!
Has any of you two tried ATACseq yet and if yes do you see a nucleosomal-like pattern after tagmantation? In our hands we only see a smear.
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Old 03-05-2015, 09:00 AM   #4
Agun
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No, It seems a little bit complicated without having seen anybody do it.

What is your experience?
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