Hi all,
When I was using the RSEM to align my reads.fastq to my transcripts.fasta (trinityrnaseq_r2013-02-25/util/RSEM_util/run_RSEM_align_n_estimate.pl), I happened to see this:
bowtie -q --phred33-quals -n 2 -e 99999999 -l 25 -I 1 -X 1000 --chunkmbs 200 -p 4 -a -m 200 -S TRANS -1
But my fastq used phred64, so is there a way to change the default setting (phred33)? Does it affect much if I used the default setting?
Thanks,
He
When I was using the RSEM to align my reads.fastq to my transcripts.fasta (trinityrnaseq_r2013-02-25/util/RSEM_util/run_RSEM_align_n_estimate.pl), I happened to see this:
bowtie -q --phred33-quals -n 2 -e 99999999 -l 25 -I 1 -X 1000 --chunkmbs 200 -p 4 -a -m 200 -S TRANS -1
But my fastq used phred64, so is there a way to change the default setting (phred33)? Does it affect much if I used the default setting?
Thanks,
He
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