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Old 08-10-2010, 05:16 AM   #1
cbrennan
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Location: Ann Arbor

Join Date: Dec 2008
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Default OLB 1.6.1 vs 1.8.0

Hello all,

We started using OLB-1.8.0 last week, I've been running some tests. For genomic samples it looks great, an increase in clusters detected, but same high pass filter & mapping rates. However for mRNA & CHiP-Seq samples I'm seeing a decrease in called clusters for half the samples -- has anyone else noticed this? I'm wondering if 1.8.0 is something we should only be using for genomic and stick to 1.6.1 for other types of samples...thoughts?
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Old 08-10-2010, 06:35 AM   #2
hoisinjl
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Default

The only thing we noticed when doing comparisons of 1.8 and 1.6 was that when cluster density was below 200,000 clusters per tile (~350,000 clusters/mm2) that there was either a small increase or a decrease in the number of reads. In all samples with a higher density, we saw at least a 10% increase, including RNA and ChiP-seq samples.
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