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Old 08-10-2010, 05:16 AM   #1
Location: Ann Arbor

Join Date: Dec 2008
Posts: 28
Default OLB 1.6.1 vs 1.8.0

Hello all,

We started using OLB-1.8.0 last week, I've been running some tests. For genomic samples it looks great, an increase in clusters detected, but same high pass filter & mapping rates. However for mRNA & CHiP-Seq samples I'm seeing a decrease in called clusters for half the samples -- has anyone else noticed this? I'm wondering if 1.8.0 is something we should only be using for genomic and stick to 1.6.1 for other types of samples...thoughts?
cbrennan is offline   Reply With Quote
Old 08-10-2010, 06:35 AM   #2
Location: St. Louis, MO

Join Date: Jun 2009
Posts: 23

The only thing we noticed when doing comparisons of 1.8 and 1.6 was that when cluster density was below 200,000 clusters per tile (~350,000 clusters/mm2) that there was either a small increase or a decrease in the number of reads. In all samples with a higher density, we saw at least a 10% increase, including RNA and ChiP-seq samples.
hoisinjl is offline   Reply With Quote

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