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Old 05-17-2016, 08:40 AM   #1
Bioo Scientific
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Location: Austin, Tx

Join Date: Oct 2009
Posts: 99
Default Using the NEXTflex Small RNA-Seq Kit v3 for RIP-Seq, CLIP-Seq and Ribosomal Profiling

A number of our customers have asked for a protocol which would enable them to use the NEXTflex™ Small RNA Seq Kit v3 for ribosomal profiling, RIP-Seq (RNA binding protein immunoprecipitation and sequencing), and CLIP-Seq (cross-linking immunoprecipitation and sequencing) experiments. To use the NEXTflex Small RNA-Seq Kit V3, the RNA of interest must have a 5' monophosphate and 3' hydroxyl. Using the following steps posted on our website, these modifications can easily be added to most RNA molecules with T4 polynucleotide kinase (PNK).
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