SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
New! Ovation RNA-Seq System V2 from NuGEN Technologies NuGEN Vendor Forum 2 07-21-2011 08:46 PM
Experience with nugen ovation 3 dge System moritzhess Sample Prep / Library Generation 0 11-06-2010 01:38 PM
New! Ovation WGA FFPE and RNA-Seq FFPE workflow solutions from NuGEN Technologies NuGEN Vendor Forum 0 11-03-2010 09:02 AM
Intron spikes in Nugen Ovation data? Scoob RNA Sequencing 1 10-15-2010 04:04 PM
RNA-Seq: Comparison between NuGEN's WT-Ovation Pico and One-Direct Amplification Syst Newsbot! Literature Watch 2 10-04-2010 08:36 AM

Reply
 
Thread Tools
Old 02-08-2011, 10:35 AM   #1
pbluescript
Senior Member
 
Location: Boston

Join Date: Nov 2009
Posts: 224
Default Alternatives to Nugen Ovation RNA-Seq

Has anyone used an alternative to the Nugen Ovation RNA-Seq kit? Specifically, I'm looking for something with similar amplification capabilities, but with only random hexamers for the amplification instead of a mixture of random hexamers and oligo-dT primers.
pbluescript is offline   Reply With Quote
Old 02-13-2011, 01:14 PM   #2
pbluescript
Senior Member
 
Location: Boston

Join Date: Nov 2009
Posts: 224
Default

Bump with more questions.
Has anyone obtained RNA-Seq data from the Nugen kit? Do you see a significant 3' bias? Any suggestions about how to deal with it either with another sequencing experiment or during analysis?
pbluescript is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 09:50 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO