Tweet
Originally posted by Richard Finney
View Post
-
They modified the settings to make sure lots of valid alignments are not reported, just like (bowtie -m 1 -v 3). Then they use a better aligner and find more usable reads. Some interesting things about contaminants and quite ambitious to reanalyze all datasets but why not use a better aligner in the first place. -
Well, it's very arguable that Shrimp2 is "better" than BWA/Bowtie2 (whatever that means).
Thank you for the paper though, definitely interesting take on the problem.Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment