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  • EnsEMBL variant effect predictor standalone error

    Hi all,

    I have extracted variation from bam files using samtools, now i want to annotate it using variant effect predictor perl script of EnsEMBL,

    [orf@localhost snp_effect_predictor]$ perl variant_effect_predictor.pl -i ../samtools-0.1.12a/samfiles/q20/vcf/GKUNU9Q04_chr1_q20_sort.vcf -format vcf -o chr1_.txt -w -b 2000

    it did well but applying for next time for another vcf file, i get error

    [orf@localhost snp_effect_predictor]$ perl variant_effect_predictor.pl -i ../samtools-0.1.12a/samfiles/q20/vcf/GKUNU9Q04_chr2_q20_sort.vcf -format vcf -o chr2_.txt -w -b 2000

    -------------------- EXCEPTION --------------------
    MSG: Start must be less than or equal to end+1
    STACK Bio::EnsEMBL::Feature::new /home/orf/EnsEMBL/src/ensembl/modules/Bio/EnsEMBL/Feature.pm:139
    STACK Bio::EnsEMBL::Variation::VariationFeature::new /home/orf/EnsEMBL/src/ensembl-variation/modules/Bio/EnsEMBL/Variation/VariationFeature.pm:177
    STACK toplevel variant_effect_predictor.pl:304
    Ensembl API version = 61
    ---------------------------------------------------

    It says there's may be a problem in vcf file but it worked well for GKUNU9Q04_chr1_q20_sort.vcf. I have used same commands for making vcf file for all chromosomes FYI.
    Am i doing anything wrong?

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