Tweet
We are using the Chemagen system (http://www.chemagen.com/fileadmin/do...n_brochure.pdf) to extract total nucleic acid from blood and stool samples. The process yields a mixture of purified DNA/RNA, but doesn't suggest how to separate the two. Has anyone done this type of separation before? I know Qiagen uses columns in their AllPrep system, but they can't be purchased separately. Most RNAse or DNase protocols I see are designed to remove small amounts of contaminating RNA/DNA, I don't know how they'd do with total nucleic acid?
-
-
If you need both RNA & DNA from the same prep, then split your prep in half. Treat one half with DNase, then run it over a column (qiagen/zymo whatever) to clean up. Base hydrolyze the other half, then EtOH precipitate. -
Thank you very much, that's likely how I'll proceed. I was going to use RNase A, but base hydrolysis is likely more cost effective, especially for a lot of samples.Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment