Hi all,
I recently did a MiSeq run that failed before it had really begun. I am doing 16S custom sequencing with the barcodes.
Everything was properly calculated, diluted and Qubited, so I am sure the right concentrations were loaded (went for 8pM) and 10% Phi X.
The only thing we can think it might have been is that some chlorine or other inhibitors may have got through and ruined the run.... Does anyone have any ideas about this or any experience with inhibitory samples?
thanks
I recently did a MiSeq run that failed before it had really begun. I am doing 16S custom sequencing with the barcodes.
Everything was properly calculated, diluted and Qubited, so I am sure the right concentrations were loaded (went for 8pM) and 10% Phi X.
The only thing we can think it might have been is that some chlorine or other inhibitors may have got through and ruined the run.... Does anyone have any ideas about this or any experience with inhibitory samples?
thanks
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