I am looking for the sequences of the sRNA3', sRNA 5', and SRA Rt primer mentioned in the Illumina Directional mRNA seq adaptor. These sequences are listed in the main "sequence" thread but I noticed the the sRNA 3' listed there lacks the 5' adenylation crucial for ligation using the truncated RNA pol.
Also, does anyone know if these oligos have to be RNA (or have to containe Uracil)? Seems like it is safer and cheaper to make them DNA.
Thanks!
Also, does anyone know if these oligos have to be RNA (or have to containe Uracil)? Seems like it is safer and cheaper to make them DNA.
Thanks!
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