Dear all,
I am currently designing an experiment, where my samples will have both a barcode and an index.
Barcode: Sample-specific
Index: Reaction-specific (here: ChIP reaction)
So I might have X samples with each a unique barcode pooled together with the same index (A). And then Y samples with each a unique barcode pooled together with the same index (B).
Can anyone advice me on the best way to perform demultiplexing on this type of data?
I will specify the index on the sample sheet (here A or B), but then how to get the reads that are sample-specific, that is what I don't know how to do.
I am hoping to get help to find an established tool for this.
(sequencing will be done on MiSeq, HiSeq and/or NovaSeq)
I am currently designing an experiment, where my samples will have both a barcode and an index.
Barcode: Sample-specific
Index: Reaction-specific (here: ChIP reaction)
So I might have X samples with each a unique barcode pooled together with the same index (A). And then Y samples with each a unique barcode pooled together with the same index (B).
Can anyone advice me on the best way to perform demultiplexing on this type of data?
I will specify the index on the sample sheet (here A or B), but then how to get the reads that are sample-specific, that is what I don't know how to do.
I am hoping to get help to find an established tool for this.
(sequencing will be done on MiSeq, HiSeq and/or NovaSeq)
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