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Old 02-20-2019, 05:45 AM   #1
anjama
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Default Agilent SureSelect XT and Illumina flowcell saturation

Does anyone know if the SureSelect XT kits have enough probes in a single reaction to saturate a single lane in an Illumina HiSeq flowcell (200-400 million reads) with unique fragments? My concern is that if it doesn't, then my sequencing will consist of excessive pcr duplicates. Thanks.
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