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I have moved to trimmomatic - it does SE and PE as well and in my hands much much faster.
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FAR does SE/PE as well (starting from version 2 AFAIK).Originally posted by kga1978 View Post
Speed is not really a matter for me. If it takes five minutes per lane, fine, if it takes one hour, fine too (considering instrument run time and time for downstream analysis) :-)
Have you compared the results? Similar?Comment
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Yes, parameters and/or working principles are poorly documented. I agree, it is really fast.Originally posted by dawe View Post
Are you using fastq-mcf in production?Comment
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I'm using fastq-join and fastq-mcf. The first for overlapping paired end reads, the second to de-multiplex NuGEN RNA-seq libraries.Originally posted by sklages View Post
I'm trying to get some more doc (the developer is very active...)Comment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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