I have multiple fastq files generated by sequencing single end library on multiple lanes on Illumina GA. I am not sure how to merge them to generate a consolidate reference genome alignment file using Tophat.
If I had multiple fastq files for paired end reads, I would have run TopHat by providing two comma separated lists of files to TopHat. But I am not sure if I can do that with single end reads as well.
Any thoughts?
Thanks.
If I had multiple fastq files for paired end reads, I would have run TopHat by providing two comma separated lists of files to TopHat. But I am not sure if I can do that with single end reads as well.
Any thoughts?
Thanks.
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