Has anyone automated any or all of the Illumina library prep steps on a robot? Beckman, Tecan, Hamilton? Any help will be appreciated. Thanks
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Same here
Looking for Library prep automation to increase throughput and eliminate variations caused by different users. In addition to the automation mentioned above from BIG_SNP, are there any other new instrumentation for full automation, say with thermal blocks?
Comment
-
Originally posted by Michael L. Altshuler View PostSPRIworks Fragment Library System I and II are claimed to be the walk away automates for the library preparation (Beckman Genomics). I would like to know what they actually do, especially the start point (blood?) and endpoint (Adaptor Ligation???) for Illumina library
Comment
-
We are currently trying to automate as much of the set up as possible. We are using DNA fragmentase to fragment the DNA and think we can automate a number of the subsequent ligation and adapter annealing steps, although this involves manually placing plates onto PCR blocks. We are going to be using the ampure xp for the PCR clean up steps and dont envisage that being problematic. Finally we are using nimblegen sequence capture in solution and have a protocol for that but we have not run any samples yet. We are going to use a beckman Fx for the pre-PCR steps and a beckman Nx for the post-PCR steps and we are aiming to run a test batch of samples in the next 2 weeks once all our reagents arrive. I'd be happy to pass on details when we have an optimised pipeline.
Comment
-
Hi, I can confirm what James said above. The Sanger use a Biomek FX and a Biomek NX to automate their library prep for the Illumina. Beckman also have another site in the UK automating 96 library preps at a time on the Biomek FX with qPCR set up done on a Biomek 3000.
Comment
Latest Articles
Collapse
-
by seqadmin
The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...-
Channel: Articles
05-06-2024, 07:48 AM -
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
04-22-2024, 07:01 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Yesterday, 06:57 AM
|
0 responses
11 views
0 likes
|
Last Post
by seqadmin
Yesterday, 06:57 AM
|
||
Started by seqadmin, 05-06-2024, 07:17 AM
|
0 responses
16 views
0 likes
|
Last Post
by seqadmin
05-06-2024, 07:17 AM
|
||
Started by seqadmin, 05-02-2024, 08:06 AM
|
0 responses
19 views
0 likes
|
Last Post
by seqadmin
05-02-2024, 08:06 AM
|
||
Started by seqadmin, 04-30-2024, 12:17 PM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
04-30-2024, 12:17 PM
|
Comment