Question Re:Sequence Attachment to Illumina Flow Cell

curran · 04-23-2009, 01:52 PM

Does anyone know how sequences are actually attached to the Illumina flow cell prior to amplification? Is it a splint ligation? The only answer I can get out of Illumina is that there's a "covalent modification" which facilitates attachment. Is anyone aware of what this modification is?

Thanks for the help!

james hadfield · 05-11-2009, 01:11 PM

If you mean how are the primers attached to the surface of the flow cell then I am not sure, possibly as simple as silanisation or one of the other the methods used for microarray production.
If you mean how does the single DNA lbrary molecule get attached then it is a hybridisation to the oligo on the flow cell followed by Taq extension of this oligo to produce a covalently attached copy of the original molecule. When the first bridge-amplification happens the orgnainl library molecule gets melted off.

Similar Threads
Thread	Thread Starter	Forum	Replies	Last Post
Frozen flow cell	Meliss a	General	4	03-06-2019 06:42 AM
what specific sequence is used for annealing to flow cell?	ein_io	Illumina/Solexa	3	12-13-2011 08:56 AM
Flow cell modifications ?	JYC	Illumina/Solexa	2	04-08-2011 11:52 AM
Method to reuse Illumina flow cell	cybog337	Illumina/Solexa	5	08-24-2010 06:26 PM
sequences on the flow cell	suludana	Illumina/Solexa	0	07-22-2008 08:51 AM

04-23-2009, 01:52 PM	#1
curran Junior Member Location: north carolina Join Date: Apr 2009 Posts: 1	Question Re:Sequence Attachment to Illumina Flow Cell Does anyone know how sequences are actually attached to the Illumina flow cell prior to amplification? Is it a splint ligation? The only answer I can get out of Illumina is that there's a "covalent modification" which facilitates attachment. Is anyone aware of what this modification is? Thanks for the help!

05-11-2009, 01:11 PM	#2
james hadfield Moderator Cambridge, UK Community Forum Location: Cambridge, UK Join Date: Feb 2008 Posts: 221	If you mean how are the primers attached to the surface of the flow cell then I am not sure, possibly as simple as silanisation or one of the other the methods used for microarray production. If you mean how does the single DNA lbrary molecule get attached then it is a hybridisation to the oligo on the flow cell followed by Taq extension of this oligo to produce a covalently attached copy of the original molecule. When the first bridge-amplification happens the orgnainl library molecule gets melted off.