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Old 03-03-2021, 03:08 AM   #1
michael10275
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Location: Europe

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Default Use Illumina UDI's in a combinatorial fashion?

Hello All,

I am wondering if someone has used selected UDI sequences such as the IDT UDI 10 nt long barcodes in a combinatorial fashion and made any convincing results or would advise against doing this?

Aim would be to do this on a MiSeq, so Index hopping is not of concern. I would like to run a smallish, preliminary experiment using ~ 4x96 samples (all on one MiSeq run) but also for this specific reason I don't want to order that many UDI pairs.

Thanks!

Last edited by michael10275; 03-03-2021 at 06:27 AM.
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barcoding, combinatorial, index, plexity, udi

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