Metagenomic WGS and Individual genome

[gauravamit]

I have Metageome WGS data? I want to know may I assemble my individual genomic sequence from it and do the assembly and annotation studies? If yes how?
I have used CLC Bio to do the assembly. What can I used to do such type of work? How much significant would it be?

[jimmybee]

Metagenomics assembly of WGS data is quite technical so you should probably have a look at a few papers and assess what assemblers they used and what information they had. From my own standpoint, the metagenomics assembly program "MetaVelvet" (http://metavelvet.dna.bio.keio.ac.jp/) and the Human Microbiome project (https://commonfund.nih.gov/hmp/) are two good sources of information on where to start. The recent Nature papers on HMP have good methods to have a look at.

I havent heard anyone using CLC-bio for metagenomics WGS assembly, so you may need to investigate a open-source assembler such as metaIDBA.

Good luck