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I want to do chip-seq for a transcription factor in bacteria for Illumina. I' need help for sample preparation for bacteria since all I find is eukaryotes. Brand new with the technique, sorry!
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I haven't done ChIP in bacteria but once you have the ChIP material I would think making the libraries would be the same. I'm waiting to get back sequencing data from my first ChIP seq libraries. If all looks good then I will be able to give some advice. You could contact Illumina to see if they have any protocols for ChIP in prokayotes. -
A very interesting study shows some of the pitfalls of ChIP applications and offers some advances to published protocols.
The paper is dedicated to ChIP on Chip but most of it also applies to Chip-Seq, too.
If you're new to the tech, I would recommend reading this paper.
Here's the ref: Waldminghaus & Skarstad 2010, BMC Genomics, 11:414Comment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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